BM6021P Catenin alpha-3 antibody
see related secondary antibodiessee all 4 Catenin alpha-3 products
0.1 mg / US$ 445
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Catenin alpha-3 892_24D2S
Synonyms
CTNNA3, Alpha T-catenin, , , Cadherin-associated protein
Product review
Please read our product review about Catenin alpha-3 : Antibodies to Catenins.
Product Description
Mouse anti Catenin alpha-3 892_24D2S, Presentation: Purified. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | 892_24D2S |
| Catalog Number | BM6021P |
| Swiss Prot. No. | Q9UI47 |
| Quantity | 0.1 mg |
| Price | US$ 445 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM6021P.pdf |
Datasheet Extract
| Monoclonal Antibody to alpha T-Catenin | |
| Concentration | 1.0 mg/ml |
| Background | Catenins are proteins that connect cadherin/β- catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with αT-catenin cDNA, interaction with β-catenin was demonstrated by coimmunoprecipitation. |
| Immunogen | Synthetic peptide corresponding to amino acids 164-177 of the human αT-catenin protein including an additional N-terminal cysteine (CVANKSDLQKTYQKL) coupled to keyhole limpet hemocyanin. Swiss Num.: Q9UI47 |
| Format | State: Liquid purified IgG fraction. BufferSystem: PBS with 0.09% Sodium Azide as preservative. |
| Applications | Immunofluorescence: aceton-fixed cells.
Immunocytochemistry: methanol and paraformaldehyde-fixed cells, followed by permeabilisation in 0.2% Triton-X 100 for 2 min. Immunohistochemistry1: 1/25-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, Immunoprecipitation. |
| Specificity | The 892_24D2S antibody against αT-catenin does not show any cross reaction with αE-catenin or αNcatenin in western blot analysis.
This antibody reacts with an epitope located between residues 164-177 of the human αT-catenin protein. Species: Human. |
| Storage | Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch. |
| References | 1. Janssens, B., Goossens, S., Staes, K., Gilbert, B., van Hengel, J., Colpaert, C., Bruyneel, E., Mareel, M., and van Roy, F. (2001). alphaTcatenin: a novel tissue-specific beta-cateninbinding protein mediating strong cell-cell adhesion, J. Cell. Sci. 114, 3177-88.
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM6021P Catenin alpha-3 '.