BM5060 Estrogen Receptor alpha / ESR1 antibody
see related secondary antibodiessee all 119 Estrogen Receptor alpha / ESR1 products
1 ml / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Estrogen Receptor alpha / ESR1 LH01
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Mouse anti Estrogen Receptor alpha / ESR1 LH01, Presentation: Supernatant. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Supernatant |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | LH01 |
| Catalog Number | BM5060 |
| Swiss Prot. No. | P03372 |
| Quantity | 1 ml |
| Price | US$ 375 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM5060.pdf |
Datasheet Extract
| Immunogen | Synthetic peptide Swiss Num.: P03372 |
| Format | State: Liquid Culture supernatant contains 0.1% NaN3 |
| Applications | Immunocytochemistry.
Immunohistochemistry on frozen sections: 1:5 - 1:10. Immunohistochemistry on paraffin sections (after microwave pretreatment): 1:5 - 1:10. Recommended Positive Control: ER-positive cell line. |
| Specificity | Estrogen receptor can be detected in up to 70% of breast carcinomas. The quantity of estrogen receptor protein is important for prognosis and treatment of breast cancer (e.g. response to endocrine therapy). The antibody shows nuclear, no cytoplasmic staining. Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Clark GM et al. Clinical usefulness of oestrogen-receptor and other markers of hormone dependence. Proc Royal Society Edinburgh. 95B:145-150 (1989)
2. Mason BH et al. Progesterone and Oestrogen Receptor as Prognostic Variables in Breast Cancer (1993). Cancer Research 43, 2985-2990 (1983) |
| Protocols | Application on paraffin sections
- Fixation: Small tissue blocks should be fixed as soon as possible after removal, in 10% buffered formalin. The fixation time should not exceed 24 hours. During the embedding process temperatures must be kept below 60°C. - Mount sections on slides coated with an appropriate medium (Vectabond, APES) at 37°C overnight followed by drying at 56°C for 60 minutes. - Deparaffinize sections and rehydrate to distilled water. Antigen retrieval - Place slides in a coplin jar containing 0.01 M sodium citrate buffer, pH 6.0. - Incubate for 3 x 5 minutes in a microwave oven (750W) with 1 min intervals. - Quench sections for 30 min at room temperature. - Rinse for 30 min in PBS. - Block endogeneous peroxidase activity (0.3% H2O2 in 50% methanol). - Apply (approximately diluted) Receptor Antibody. - Continue immunohistochemical staining procedure with conjugate and substrate reaction. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM5060 Estrogen Receptor alpha / ESR1 '.
