BM5059 Androgen Receptor / AR antibody
see related secondary antibodiessee all 36 Androgen Receptor / AR products
1 ml / US$ 445
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Androgen Receptor / AR F39.4
Synonyms
Dihydrotestosterone Receptor, DHTR, NR3C4, , Nuclear receptor subfamily 3 group C member 4, testicular feminization, spinal and bulbar muscular atrophy, Kennedy disease, AIS, HUMARA, KD, SBMA, SMAX1, TFM
Product review
Please read our product review about Androgen Receptor / AR : Antibodies to Androgen Receptor.
Product Description
Mouse anti Androgen Receptor / AR F39.4, Presentation: Supernatant. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Supernatant |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | F39.4 |
| Catalog Number | BM5059 |
| Swiss Prot. No. | P10275 |
| Quantity | 1 ml |
| Price | US$ 445 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM5059.pdf |
Datasheet Extract
| Background | The androgen receptor (AR) has been localized e.g. in the testis, (Sertoli and Leydig cells), prostate, foreskin epidermis, fibroblasts, breast epithelium, sweat and apocrine glands and hepatocytes.
Prostate cancers are very heterogeneous with regard to AR expression, the loss of androgen responsiveness is not paralleled by the loss of AR. Studies on AR distribution might be useful to study the role of AR in related disease processes. |
| Immunogen | Synthetic peptide (aa 301-320 of human AR)
Swiss Num.: P10275 |
| Format | State: Liquid purified IgG, containing 0.09% sodium azide |
| Applications | Western blotting.
Immunocytochemistry. Immunohistochemistry on frozen and paraffin embedded sections (microwave pretreatment): 1/5-1/10. Dilute immediately before use with PBS. Recommended positive control: Testis. |
| Specificity | The antibody is reactive with a 110 kD protein in a lysate from the LNCP cell line.
Antigen localization is nuclear. Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Hinchliffe SA, Woods S, Gray S, Burt AD: Cellular distribution of androgen receptors in the liver. J Clin Pathol 49, 418-420 (1996)
2. Brooks BP, Merry DE, Paulson HL, Lieberman AP, Kolson DL, Fischbeck KH: A cell culture model for androgen effects in motor neurons. J Neurochem 70, 1054-1060 (1998) 3. Jansen PJ et al. Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded tissue after microwave pretreatment. J Histochem Cytochem 42,1169-1175 (1994) 4. Ruizeveld de Winter JA et al. Androgen receptor expression in human tissues: an immunohistochemical study. J Histochem Cytochem 39, 927-936 (1991) 5. Zegers ND et al. Epitopic prediction and confirmation for the human androgen receptor. Generation of monoclonal antibodies for multi-assay performance following the synthetic peptide strategy. Biochem Biophys Acta 1073, 23-32 (1991) |
| Protocols | Application on paraffin sections
- Fixation: Small tissue blocks should be fixed as soon as possible after removal, in 10% buffered formalin. The fixation time should not exceed 24 hours. During the embedding process temperatures must be kept below 60°C. - Mount sections on slides coated with an appropriate medium (Vectabond, APES) at 37°C overnight followed by drying at 56°C for 60 minutes. - Deparaffinize sections and rehydrate to distilled water. Antigen retrieval - Place slides in a coplin jar containing 0.01 M sodium citrate buffer, pH 6.0. - Incubate for 3 x 5 minutes in a microwave oven (750W) with 1 min intervals. - Quench sections for 30 min at room temperature. - Rinse for 30 min in PBS. - Block endogeneous peroxidase activity (0.3% H2O2 in 50% methanol). - Apply prediluted receptor antibody. - Continue immunohistochemical staining procedure with conjugate and substrate reaction. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM5059 Androgen Receptor / AR '.
