BM4025X S100A8+S100A9 / Calgranulin A+B / MRP8+MRP14 antibody
see related secondary antibodiessee all 8 S100A8+S100A9 / Calgranulin A+B / MRP8+MRP14 products
1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti S100A8+S100A9 / Calgranulin A+B / MRP8+MRP14 27E10
Synonyms
S100-A8, S100-A9, S100, CFAG, P8, P14, CAGA, CAGB, CFAG, MRP-8, MRP-14, , Migration inhibitory factor-related protein 14, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit, Calgranulin B, Migration inhibitory factor-related protein 8, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Calprotectin L1L subunit, Urinary stone protein band A
Product review
Please read our product review about S100A8+S100A9 / Calgranulin A+B / MRP8+MRP14 : Antibodies to S100 Family.
Product Description
Mouse anti S100A8+S100A9 / Calgranulin A+B / MRP8+MRP14 27E10, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 27E10 |
| Catalog Number | BM4025X |
| Swiss Prot. No. | P06702 |
| Quantity | 1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4025X.pdf |
Datasheet Extract
| Alternate names | Calprotectin, L1 protein |
| Concentration | 6.3 mg/ml (by OD280nm) |
| Background | The antigen is produced by the heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.
|
| Immunogen | Swiss Num.: P06702 |
| Format | State: Liquid Purification: Protein G affinity chromatography BufferSystem: PBS, pH 7.2, containing 0.09% sodium azide |
| Applications | Immunohistochemistry on acetone fixed frozen sections and formalin fixed paraffin sections(1 µg/ml). Predigestion with Trypsin or Proteinase K is required.
ELISA. |
| Specificity | Specific for a subpopulation of macrophages, monocytes and granulocytes; peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly.
27E10 is a unique monoclonal antibody that recognizes an epitope on the MRP8/14 heterocomplex that is not exposed on the individual subunits MRP8 or MRP14. The antibody is ideally suited for the detection of early inflammatory macrophages and therefore for the classification of acute stage inflammation in tissue sections and in smears, the characterization of tumorous tissues and the in vitro monitoring of peripheral blood cell cultures. The MRP8/14 specific ELISA has shown unprecedented advantages in the early assessment of acute inflammatory conditions like transplant rejection. Species: Human. Stains a subpopulation of macrophages in rhesus monkey. |
| Add. Information | Antigen distribution in isolated cells:
A subpopulation of monocytes and neutrophilic granulocytes are positive. Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80% of monocytes in early cultures (24-48h) are positive. No reaction has been seen with lymphocytes or platelets. Antigen distribution in tissue sections: Macrophages in the red pulp of the spleen and in the liver; strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen; absent on normal resident mononuclear phagocytes in healthy tissues (skin, gut, thymus). |
| Storage | Stock solution or aliquots thereof can be kept frozen at -20°C. Avoid repeated freezing and thawing.
Do not freeze working dilutions. Shelf life: one year from despatch. |
| References | Zwadlo, G. et al.: A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues. J. Immunol. 137: 512-518 (1986)
Steinhoff, G. et al.: Patterns of macrophage immigration and differentiaton in human liver grafts. Transplant. Proc. 21(1), 398 - 400 (1989). Johne, B. et al.: Functional and clinical aspects of the myelomonocyte protein calprotectin. J. Clin Pathol. 50: 113-123 (1997). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
