BM4019 Ly-6C (Macrophage precursor cells) antibody
see related secondary antibodiessee all 3 Ly-6C products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Ly-6C (Macrophage precursor cells) ER-MP20
Synonyms
Mouse Macrophage Marker
Product Description
Rat anti Ly-6C (Macrophage precursor cells) ER-MP20, Presentation: Purified. Product is tested for Frozen sections ( C ), Paraffin Sections ( P ), Flow cytometry ( F )
Properties
| Applications | Frozen sections ( C ), Paraffin Sections ( P ), Flow cytometry ( F ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rat |
| Isotype | IgG2a |
| Clone | ER-MP20 |
| Catalog Number | BM4019 |
| Quantity | 0.1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4019.pdf |
Datasheet Extract
| Monoclonal Antibody to //Mouse Mid-Stage Macrophage Precursor Cells | |
| Alternate names | Ly-6C, Late CFU-M, Monoblasts and Monocytes |
| Concentration | 0.2 mg/ml |
| Background | The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 14 kD surface protein which is very similar to Ly-6C and may be analogous to human CD59.
It is inducible by IFN-alpha, IFN-beta and IFN-gamma. |
| Immunogen | Mouse macrophage cell lines. |
| Format | State: Lyophilized purified Ig fraction Purification: Affinity chromatography BufferSystem: PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% thimerosal as a preservative. Reconstitution: Restore with 0.5 ml distilled water. |
| Applications | Immunohistochemistry on frozen sections: 0.5 µg/ml (1/400); Fixation: Acetone and paraffin sections: 1 µg/ml (1/200). Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Mouse spleen. Has been described to work in FACS. Antigen distribution on Isolated cells and Tissue sections: In bone marrow cells the antigen is found on monoblasts and late CFU-M cells as well as on monocytes. It is also found on granulocytes and a subpopulation of lymphocytes in the peripheral blood. Granulocytic cells show a dull, and monocytic cells a bright antigen surface expression. Lymphoid cells express the antigen only very weakly. Thus, in the bone marrow three useful FACS windows can be defined for cell sorting purposes. The antigen is found on macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs also stain positive with ER-MP 20. Activated macrophages in inflammatory tissues also express the ER-MP 20-related antigen. Antibody BM4019 reacts with mouse macrophage precursor cells, other species not tested. |
| Specificity | ER-MP 20 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ER-MP 20 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM 8 (anti F4/80) is recommended. ER-MP 20 also detects a wide range of endothelial cells. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. DE BRUIJN M.F.T.R., et al.: Distinct mouse bone marrow macrophage precursors identified by differential expression on the ER-MP12 and ER-MP20 antigens. Eur.J. Immunol. 24, 2279-2284 (1994).
2. DE BRUIJN M.F.T.R., et al. Analysis of ER-MP12/20 bone marrow populations in Listeria monocytogenes infected mice: a flow cytometric alternative for differential counting. J Immunol Meth. In press. (1998). 3. CHAN J., et al. Macrophage lineage cells in inflammation: characterization by CSF-1 receptor (c-Fms), ER-MP58 and ER-MP20 (Ly-6C) expression. Blood, in press (1998). 4. McCORMACK J., et al. Macrophage Progenitors from Mouse Bone Marrow and Spleen differ in their Expression of the Ly-6C Differentiation Antigen. J. Immunol. 151(11), 6389-6398 (1993). 5. P.J.M. LEENEN et al.: Murine Macrophage Precursor Characterization II. Monoclonal Antibodies against Macrophage Precursor Antigens. Eur. J. Immunol. 20, 27-34 (1990). 6. P.J.M. LEENEN et al.: Murine Macrophage Precursor Characterization I. Production, phenotype and differentiation of macrophages precursor hybrids J. Immunol. 20, 15-25 (1990). 7. R.H.J. BEELEN et al.: Milky spots in the omentum play an important role in the origin and differentiation of peritoneal macrophages. Abst. Vth annual conference of the Upper Rhine Universities on the Macrophage, Sept. 4/5th (1991). 8. P.J.M. LEENEN et al.: Differential Inhibition of Macrophage Proliferation by Anti-Transferin Receptor antibody ER-MP 21: Correlation to Macrophage Differentiation Stage. Exp. Cell Res. 189, 55-63 (1990). |
| Protocols | Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS 6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS 13. Counterstain. |
| Pictures | Figure 1. Immunohistochemistry on Mouse spleen frozen sections using antibody BM4019. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170,00 € | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 210,00 € | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'BM4019 Ly-6C (Macrophage precursor cells)'.
