BM4008LE Macrophages (F4/80 antigen) antibody
see related secondary antibodiessee all 61 Macrophages products
0.5 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Macrophages (F4/80 antigen) Cl:A3-1
Synonyms
Mouse Macrophage Marker, Pan macrophages, ,
Product review
Please read our product review about Macrophages (F4/80 antigen): Antibodies to Mouse Macrophages.
Product Description
Rat anti Macrophages (F4/80 antigen) Cl:A3-1, Presentation: Low Endotoxin. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Functional assay ( FN ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Radioimmunoassay ( R ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Functional assay ( FN ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Radioimmunoassay ( R ), Western blot / Immunoblot ( WB ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Low Endotoxin |
| Host | Rat |
| Isotype | IgG2b |
| Clone | Cl:A3-1 |
| Catalog Number | BM4008LE |
| Quantity | 0.5 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4008LE.pdf |
Datasheet Extract
| Monoclonal Antibody to Mouse F4/80 Antigen // Low Endotoxin | |
| Concentration | 1.0 mg/ml |
| Background | F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells. |
| Immunogen | Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line. |
| Format | State: Liquid purified IgG Purification: Affinity chromatography on Protein G BufferSystem: PBS, pH7.4 |
| Applications | Flow Cytometry: 1/100 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on Frozen sections. Immunohistochemistry on Paraffin sections: requires pre-treatment prior to staining; Proteinase K is recommended for tissues fixed for less than 24 hours; Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Immunohistochemistry on Resin sections. Immunoprecipitation. Western Blot. Radioimmunoassays. Functional Assays. |
| Specificity | This antibody recognises the F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes. Species: Mouse. |
| Add. Information | Endotoxin Level: less than 0.01 EU/µg |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Austyn, J.M. and Gordon, S. (1981) F4/80 , a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805 - 815.
2. Hume, D.A., Perry, V.H., and Gordon, S. (1984) The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen F4/80: Macrophages Associated with Epithelia. The Anatomical Record 210: 503 - 512. 3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80. J. Exp. Med. 161: 475 - 489. 4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues. Curr. Top. Micro. Immunol. 181: 1 - 37. 5. Warschkau, H. and Kiderlen, A.F. (1999) A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes. J. Immunol.163:3409 - 3416. 6. His-Hsien, L. et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance. J. Exp. Med. 201:1615 - 1625. |
| Pictures | Staining of J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN:Low Endotoxin |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM4008LE Macrophages (F4/80 antigen)'.