BM4008B Macrophages (F4/80 antigen) antibody
see related secondary antibodiessee all 61 Macrophages products
100 Tests / US$ 515
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Macrophages (F4/80 antigen) Cl:A3-1
Synonyms
Mouse Macrophage Marker, Pan macrophages, ,
Product review
Please read our product review about Macrophages (F4/80 antigen): Antibodies to Mouse Macrophages.
Product Description
Rat anti Macrophages (F4/80 antigen) Cl:A3-1, Presentation: Biotin. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Biotin |
| Host | Rat |
| Isotype | IgG2b |
| Clone | Cl:A3-1 |
| Catalog Number | BM4008B |
| Quantity | 100 Tests |
| Price | US$ 515 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4008B.pdf |
Datasheet Extract
| Monoclonal Antibody to Murine F4/80 Antigen - Biotin // (Mouse Macrophage Marker) | |
| Concentration | 0.1 mg/ml |
| Background | F4/80 antigen is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node.
The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells. |
| Immunogen | Thioglycollate stimulated peritoneal macrophages from C57/BL mice
|
| Format | State: Liquid purified IgG Purification: Protein G chromatography BufferSystem: PBS containing 1% BSA and 0.09% sodium azide Label: Biotin |
| Applications | Flow cytometry: Use 10 µl of neat to 1/10 diluted antibody to stain 10e6 cells in 100 µl.
Immunohistology on frozen and paraffin embedded sections. For paraformaldehyde paraffin tissue, pretreatment by trypsinisation for 20-30 minutes is required. Use 1:50 on cryosections, 1:20 on paraffin sections. Western Blot. |
| Description | CI:A3-1 recognizes the F4/80 antigen, a 120-160kD glycoprotein containing 7 EGF-like domains at the N-terminus, an RGD (Arg-Gly-Asp) integrin binding motif, and significant homology to the transmembrane 7 (Tm7) hormone receptor family at the C-terminus. The molecular structure suggests multiple ligands. Possibly, F4/80 is involved in macrophage adhesion within certain tissues, combined with receptor signaling following peptide-ligand interaction. The antigen is expressed by most macrophages and macrophage precursors beyond M-CFC, and increases upon maturation. Activated macrophages, and eosinophils express low levels. Freshly isolated spleen dendritic cells are uniformly positive for F4/80, but the expression decreases upon culture. This antibody is very useful for characterising tissue fixed macrophages, particularly in combination with BM 8 and MOMA-2. |
| Specificity | BM4008B recognizes the murine F4/80 antigen, a 160 kD cell surface glycoprotein.
|
| Add. Information | Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (ref.5). |
| References | 1. Austyn, J. M. and Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815.
2. Hume, D.A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80: Macrophages associated with epithelia. The Anatomical Record 210: 503-512. 3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80. J. Exp. Med. 161: 475-489. 4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues. Curr. Top. Micro.Immunol. 181: 1-37. 5. Warschkau, H. and Kiderlen, A.F. (1999) A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes. J. Immunol.163:3409-3416. 6. His-Hsien, L. et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance. J. Exp. Med. 201:1615-1625. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM4008B Macrophages (F4/80 antigen)'.