BM4008APC Macrophages (F4/80 Antigen) antibody
see related secondary antibodiessee all 61 Macrophages products
100 Tests / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Macrophages (F4/80 Antigen) CI:A3-1
Synonyms
Mouse Macrophage Marker, Pan macrophages, ,
Product review
Please read our product review about Macrophages (F4/80 Antigen): Antibodies to Mouse Macrophages.
Product Description
Rat anti Macrophages (F4/80 Antigen) CI:A3-1, Presentation: APC. Product is tested for Flow cytometry ( F )
Properties
| Applications | Flow cytometry ( F ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | APC |
| Host | Rat |
| Isotype | IgG2b |
| Clone | CI:A3-1 |
| Catalog Number | BM4008APC |
| Quantity | 100 Tests |
| Price | US$ 555 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4008APC.pdf |
Datasheet Extract
| Monoclonal Antibody to Mouse F4/80 Antigen - APC | |
| Immunogen | Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice |
| Format | State: Lyophilized IgG fraction Purification: Protein A affinity chromatography BufferSystem: PBS, pH 7.4, containing 0.09 sodium azide and 1% BSA Label: Allophycocyanin (APC) |
| Applications | Flow cytometry: neat - 1/10. Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl. |
| Specificity | This antibody recognises the mouse F4/80 antigen, a 160 kD glycoprotein expressed by murine macrophages.
The antibody binds to macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. The antigen is not expressed by lymphocytes or polymorphonuclear cells. |
| Storage | Prior to and following reconstitution store at 2-8°C.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Shelf life: one year from despatch. |
| References | 1. Austyn, J.M. and Gordon, S. (1981). F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815.
2. Hume, D.A. et al. (1984). The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80: Macrophages associated with epithelia. The Anatomical Record 210: 503-512. 3. Lee, S.H. et al. (1985). Quantitative analysis of total macrophage content in adult mouse tisues. Immunochemical studies with monoclonal antibody F4/80. J. Exp. Med. 161: 475-489. 4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues. Curr. Top. Microbiol. Immunol. 181: 1-37. |
| Pictures | Staining of mouse peritoneal macrophages cells with antibody BM400APC |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM4008APC Macrophages (F4/80 Antigen)'.