BM4007S Macrophages (F4/80 antigen) antibody
see related secondary antibodiessee all 61 Macrophages products
0.1 mg / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti Macrophages (F4/80 antigen) BM8
Synonyms
Mouse Macrophage Marker, Pan macrophages, ,
Product review
Please read our product review about Macrophages (F4/80 antigen): Antibodies to Mouse Macrophages.
Product Description
Rat anti Macrophages (F4/80 antigen) BM8, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rat |
| Isotype | IgG2a |
| Clone | BM8 |
| Catalog Number | BM4007S |
| Quantity | 0.1 mg |
| Price | US$ 375 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM4007S.pdf |
Datasheet Extract
| Monoclonal Antibody to //Mouse Macrophages: F4/80 Antigen //- Majority of Resident Tissue Macrophages | |
| Concentration | 0.2 mg/ml |
| Immunogen | Cultured macrophages Remarks: The F4/80 antigen is a 125 kD extracellular membrane protein sensitive to 2-mercaptoethanol. |
| Format | State: Lyophilized IgG fraction Purification: Affinity chromatography BufferSystem: PBS, pH 7.2, 5 mg/ml BSA as a stabilizer and 0.01% Kathon as a preservative Reconstitution: Restore by adding 0.5 ml distilled water. |
| Applications | Immunohistochemistry (frozen sections: 0.4 μg/ml (1:500); Paraffin sections: 4 μg/ml (1:50); Proteinase K pretreatment for antigen retrieval is recommended).
Suggested positive control: Mouse spleen. Has been described to work in FACS. |
| Specificity | This antibody is useful for the detection of the F4/80 antigen on major subpopulations of resident tissue macrophages.
The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. This clone is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to Ã?-cell destruction and diabetes in a mouse diabetes model. Species: Mouse. This antibody stains also human heart macrophages. |
| Add. Information | Antigen distribution on tissue sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.
Antigen distribution on isolated cells: expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes. |
| Storage | Store the original vial at 2-8°C. Stock solution and aliquots thereof should be stored at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Malorny, U. et al.: A monoclonal antibody angainst an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 243, 421-428 (1986).
2. Kraal, G. et al.: Macrophages in T and B Cell compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA-2, an Immunohistochemical Study. Scand. J. Immunol. 26, 653-661 (1987). 3. Leenen, P.J.M. et al.: Markers of mouse macrophage development detected by monoclonal antibodies. J. Immunol. Methods, 174: 5-19 (1994). 4. Smit, M.J. et al.: Infection of mice with lactate dehydrogenase-elevating virus destroys the subpopulation of Kupffer cells involved in receptor-mediated endocytosis of lactate dehydrogenase and other enzymes. Hepatology 12: 1192-1199 (1990) 5. Rosmalen, J.G.M. et al.: Subsets of Macrophages and Dendritic Cells in Nonobese Diabetic Mouse Pancreatic Inflammatory Infiltrates: Correlation with the Development of Diabetes. Lab. Invest. 80: 23-30 (2000) 6. Schaller, E. et al.: Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol. Cell Biol. 22: 8035-43 (2002). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM4007S Macrophages (F4/80 antigen)'.