BM2669R ABCB1 / MDR1 antibody
see related secondary antibodiessee all 15 ABCB1 / MDR1 products
100 Tests / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti ABCB1 / MDR1 UIC2
Synonyms
Multidrug resistance protein 1 P-glycoprotein 1 CD243 PGY1
Product Description
Mouse anti ABCB1 / MDR1 UIC2. Product is tested for Flow cytometry ( F )
Properties
| Applications | Flow cytometry ( F ) |
| Reactivity | Human ( Hu ) |
| Presentation | PE |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | UIC2 |
| Catalog Number | BM2669R |
| Quantity | 100 Tests |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM2669R.pdf |
Datasheet Extract
| Monoclonal Antibody to //P-glycoprotein (P-gp)- PE- | |
| Background | Multidrug resistance (MDR) is a function that renders cells resistant in a steady state way to structurally diverse lipophilic molecules (including many cytotoxic agents) after triggering by a single cytotoxic drug. One of the mechanisms that are responsible for this pleiotropic resistance effect is a reduced drug accumulation inside the cells, due to the activity of a transmembrane protein, the P-glycoprotein (P-gp). Other designations: P-170, mdr1 gene product, with a molecular weight of 170 kDa. (1)
The P-gp acts as an ATP-dependent drug efflux pump with broad substrate specificity. It expression is up-regulated when cells are treated with cytotoxic drugs. Up-regulation is linked to amplification of the mdr1 gene. MDR may also exist in numerous healthy cells and is assumed to protect them from ''naturally occurring'' drugs such as anthracyclines, vinca alkaloids, podophyllotoxins and colchicine. P-gp is mainly expressed in specialized epithelial cells with secretory or excretory functions. In the liver, P-gp is found on the biliary surface of hepatocytes and small biliary ductules; in the pancreas, on the laminal surface of the epithelial cells of small ductules and, in the kidney, on the brush border of the proximal tubules. (2) Additional sites of expression include the gastro-intestinal tract, endothelial cells of the brain as well as the adrenal glands. (3) In normal human peripheral blood lymphocytes, P-gp is expressed in the majority of CD56+ cells (NK cells). CD8+ cells (cytotoxic/suppressor T cells) and CD20+ cells (B cells) but in less than one half of CD4+ cells. (4) In normal bone marrow P-gp is detected in CD34+ hematopoietic stem cells. (1) |
| Immunogen | Balb/c 3T3-1000 cells (human MDR1 cDNA transfected Balb/c 3T3 fibroblasts). |
| Format | State: Liquid purified IgG fraction. Purification: Ion exchange or affinity chromatography. BufferSystem: 0.01 M Sodium phosphate, 0.145 M Sodium chloride, pH 7.2 containing 2 mg/ml BSA as stabilizer and 0.09% Sodium Azide as preservative. Label: conjugated to PE |
| Applications | Suitable for use in Flow Cytometry. |
| Specificity | Specifically reacts with an extracellular epitope of the human 170 kDa p-glycoprotein. |
| Storage | Store the antibodies undiluted (minimal exposure to light) at 2-8°C. DO NOT FREEZE!
Shelf life: one year from despatch. |
| References | 1. Chaudhary, P.M., et al.,(1991), Expression and activity of P-Glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells, Cell, 66, 85-94.
2. Thiebaut, F., et al.,(1987), Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues, Proc. Natl. Acad. Sci., USA, 84, 7735-7738. 3. Hunter, J., et al., (1993), Functional expression of P-glycoprotein in apical membranes of human intestinal Caco-2 cells, J. Biol. Chem., 268, 14991-14997. 4. Chaudhary, P.M., et al., (1992), Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes, Blood, 11, 80, 2735-2739. 5. Mechetner, E.B., (1992), Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody, Proc. Natl. Acad. Sci., USA, 89, 5824-5828. 6. Schinkel, A.H., et al., (1993), Binding properties of monoclonal antibodies recognizing external epitopes of the human MDR1 P- glycoprotein, Int. J. Cancer, 55, 478-484. 7. Lehne, G., et al., (1995), Binding diversity of antibodies against external and internal epitopes of the multidrug resistance gene product P-glycoprotein, Cytometry, 20. 228-237. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM2669R ABCB1 / MDR1'.
