BM2669P ABCB1 / MDR1 antibody
see related secondary antibodiessee all 15 ABCB1 / MDR1 products
100 Tests / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti ABCB1 / MDR1 UIC2
Synonyms
Multidrug resistance protein 1 P-glycoprotein 1 CD243 PGY1
Product Description
Mouse anti ABCB1 / MDR1 UIC2, Presentation: Purified. Product is tested for Flow cytometry ( F ), Frozen sections ( C ), Paraffin Sections ( P )
Properties
| Applications | Flow cytometry ( F ), Frozen sections ( C ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | UIC2 |
| Catalog Number | BM2669P |
| Quantity | 100 Tests |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM2669P.pdf |
Datasheet Extract
| Monoclonal Antibody to P-Glycoprotein (P-gp) | |
| Concentration | 0.05 mg/ml (OD280 nm, E1% = 14) |
| Background | Multidrug resistance (MDR) is a phenomenon that renders cells resistant to chemotherapy in most cancer patients. One of the mechanisms that is responsible for this pleiotropic effect is a reduced drug accumulation inside the cells, due to the activity of a transmembrane protein, the P-glycoprotein (P-gp; other designations P-170, mdr1 gene product) with a molecular weight of 170 kDa.
The P-glycoprotein is assumed to act as an ATP-dependent drug efflux pump with broad substrate specificity. Tissue Distribution: MDR mechanisms exists in numerous healthy cells and protects them from ''naturally occurring'' drugs such as anthracyclines, vinca alkaloids, podophyllotoxins and colchicine. P-gp is mainly expressed in specialized epithelial cells with secretory or excretory functions. In the liver, P-gp is found on the biliary surface of hepatocytes and small biliary ductules; in the pancreas, on the laminal surface of the epithelial cells of small ductules and, in the kidney, on the brush border of the proximal tubules. Additional sites of expression include the gastrointestinal tract, endothelial cells of the brain as well as the adrenal glands. |
| Immunogen | Balb/c 3T3-1000 cells (human MDR1 cDNA transfected Balb/c 3T3 fibroblasts). |
| Format | State: Liquid purified IgG fraction. BufferSystem: PBS containing 2 mg/ml BSA as stabilizer and 0.09% Sodium Azide as preservative. |
| Applications | Useful for Immunohistochemical techniques (both frozen and paraffin-embedded sections, 2-10 µg/ml) and Flow Cytometry (use undiluted).
It is also useful for functional experiments since UIC2 inhibits P-gp mediated efflux and potentiates the cytotoxic action of P-gp transported drugs in MDR cells. |
| Specificity | Reacts specifically with an extracellular epitope of the human 170 kDa P-glycoprotein. |
| Storage | Store the antibody undiluted (in the dark) at 2-8°C.
DO NOT FREEZE! Shelf life: one year from despatch. |
| References | 1. Mechetner, E.B., et al., (1992), Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody, Proc. Natl. Acad. Sci. 89, 8624-5828.
2. Schinkel, A.H., et al., (1993), Binding properties of monoclonal antibodies recognizing external epitopes of the human MDR1 P-glycoprotein, Int. J. Cancer, 55, 478-484. 3. Nooter, K., et al., (1991), Multidrug resistance (mdr) genes in human cancer, Br. J. Cancer, 63, 663. 4. Thibault, F., et al., (1987), Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues, Proc. Natl. Acad. Sci, 84, 7735-7738. 5. Hunter, J., et al., (1993), Functional expression of P-glycoprotein in apical membranes of human intestinal Caco-2 cells, J. Biol. Chem., 268, 14991-14997. 6. Cordon-Cardo, C., et al., (1990), Expression of the multidrug resistance gene product (P-glycoprotein) in human normal and tumor tissues, J. Histochem-Ctyochem., 38, 1277-1278. 7. Flynn, S.D., et al., (1992), P-glycoprotein expression and multidrug resistance in andrenocortical carcinoma, Surgery, 112, 981-986. 8. Chaudary, P.M., et al., (1992), Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes, Blood, 80, 2735-2739. 9. Chaudary, P.M., et al., (1991), Expression and activity of P-Glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells, Cell, 66, 85-94. 10. Goldstein, L.J., et al., (1989), Expression of a multidrug resistance gene in human cancers, J. Natl. Cancer Inst., 81, 116-124. 11. Yuen, A.R., et al., (1994), Multidrug resistance in lymphomas, J. Clin. Oncol., 12, 2453-2459. 12. Kelley, D.J., et al., (1993), Detection of P-glycoprotein in squamous cell carcinomas of the head and neck, Arch. Otolaryngol. Head Neck Surg., 119, 411-414. 13. Pavelic, Z.P., et al., (1993), Detection of P-glycoprotein with four monoclonal antibodies in normal and tumor tissues, Arch. Otolaryngol. Head Neck Surg., 119, 753-757. 14. Beck, W.T., et al., (1996), Methods to detect P-glycoprotein-associated multidrug resistance in patients' tumor: consensus recommendations, Cancer Research, 56, 3010-3020. 15. Ivy, S.P., et al., (1996), Correlation of P-glycoprotein expression and function in childhood acute leukemia: a children's cancer group study, Blood, 88, 309-318. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 € | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 € | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 € | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'BM2669P ABCB1 / MDR1'.
