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BIN023  Herpes simplex Type I (HSV) N-term Glycoprotein D Glycosylated antigen

0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Herpes simplex Type I (HSV) N-term Glycoprotein D Glycosylated

Product Description

Herpes simplex Type I (HSV) N-term Glycoprotein D Glycosylated

Properties

PresentationPurified
SourcePichia pastoris
Catalog NumberBIN023
Quantity0.1 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
BIN023.pdf

Datasheet Extract

Concentration2.0 mg/ml (Lowry)
SourcePichia pastoris (KOS strain)
Format
State: Liquid purified fraction (>95% pure by SDS-PAGE (Coomassie stain)).
Purity: Ion exchange chromatography
BufferSystem: Tris buffered saline, pH 8.0 without preservatives.
ApplicationsELISA and Western blot.
Glycosylation is heterogeneous and results in several immunoreactive products on Western blots that range from approximately 40,000 and 50,000 daltons and a doublet band at approximately 28,000 and 30,000 daltons that are not glycosylated.
DescriptionRecombinant ecto-domain of gD (amino acids 21-339). Representing the external domain of the gD molecule without membrane spanning and cytosolic sequences. It encodes a protein from 33-36 Kda that contain 3 sites for glycosylation. The yeast system adds a mannose core oligosaccharide to N-linked motifs and this may add up to 3,000-5,000 per site.
Contains N-terminal sequence YVHHHHHHLVPRGS corresponding to a polyhistidine sequence for positive control in ELISA or Western blots (required Ni-NFTA-alkaline phosphatase) and a thrombin cleavage site.
Add. InformationThis is an internal thread vial - centrifuge product before opening!
StorageAliquot and store at -70°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.