AR01011PU-S Heregulin-beta 1 (HRG1-b) antigen
see all 2 Heregulin-beta 1 products
10 µg / US$ 255
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Heregulin-beta 1 (HRG1-b)
Synonyms
Neuregulin1, NRG1-b1,
Product Description
Human Heregulin-beta 1 (HRG1-b)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >98% |
| Source | E. coli |
| Catalog Number | AR01011PU-S |
| Quantity | 10 µg |
| Price | US$ 255 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AR01011PU-S.pdf |
Datasheet Extract
| Alternate names | Neuregulin1 (NRG1-β1), Neu differentiation factor (rat) |
| Background | Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-β1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). |
| Source | E. coli |
| Format | State: Sterile filtered, lyophilized, containing no additives Purity: > 98 % by SDS-PAGE gel and HPLC analyses Endotoxinlevel: < 0.1 ng per ?g (1EU/?g). Reconstitution: Centrifuge the vial prior to opening. Restore in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for one week or at -20°C for future use. |
| Description | Recombinant human Heregulin-β1 (HRG1-β1) is a polypeptide consisting of only the EGF domain of heregulin-β1 (65 amino acid residues). AASequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE Biological Activity: The ED50 aas determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is < / = 0.5 ng/ml. Specific Activity: > / =2 x 10e6 units/mg Molecular weight: 7.5 kDa |
| Storage | The lyophilized protein is stable at -20°C for one year from date of despatch.
Reconstituted Heregulin-β1 is stable for 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freezing and thawing. |
| References | 1. Wang L, Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling Blood. ;110(12):4111-9 12/1/2007 17761519.
2. Lopez-Bendito G Tangential neuronal migration controls axon guidance: a role for neuregulin-1 in thalamocortical axon navigation. Cell.;125(1):127-42 4/7/2006 16615895. |