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<b>Figure 1.</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser315) antibody (AP02393PU).

 

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AP02393PU-N  p53 (pSer315) antibody

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see all 270 p53 products
100 µg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti p53 (pSer315)

Synonyms

TP53,

Product Description

Rabbit anti p53 (pSer315) , Presentation: Aff - Purified. Product is tested for Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsParaffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationAff - Purified
HostRabbit
Catalog NumberAP02393PU-N
Swiss Prot. No.P04637
Quantity100 µg
PriceUS$ 425
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AP02393PU-N.pdf

Datasheet Extract

Alternate namesTP53
Concentration1.0 mg/ml
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 315 (S-S-SP-P-Q).
Swiss Num.: P04637
Format
State: Liquid purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
ApplicationsSuitable for use in Western blot (1:500~1:1000) and Immunohistochemistry (1:50~1:100).
SpecificityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
p53 (phospho-Ser315) antibody detects endogenous levels of p53 only when phosphorylated at serine 315.

Species: Human.
StorageStore the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References1. Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
2. Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539.
3. Ulrich, S. J. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 5954-5958.
4. Pospísilová S, et al. (2004) Biochem J; 378(Pt 3): 939-47.
5. Merrick BA, et al. (2001) Biochemistry; 40(13): 4053-66.
PicturesFigure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser315) antibody (AP02393PU).

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'AP02393PU-N p53 (pSer315)'.