AP00816PU-N IgE antibody
see related secondary antibodiessee all 221 IgE products
1 ml / US$ 335
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Sheep anti IgE
Product Description
Sheep anti IgE , Presentation: Purified. Product is tested for Immunodiffusion ( ID )
Properties
| Applications | Immunodiffusion ( ID ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Sheep |
| Catalog Number | AP00816PU-N |
| Quantity | 1 ml |
| Price | US$ 335 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00816PU-N.pdf |
Datasheet Extract
| Concentration | 19.32mg/ml (OD280nm, E1% = 14.5) |
| Immunogen | Human IgE: IgE purified from two different myeloma sera containing IgE paraproteins representing each of the two light-chain types |
| Format | State: Liquid purified Ig (0.2µm filtered) Purification: If necessary, adsorbed to monospecificity by use of solid-phase adsorbants. Produced by ion-exchange chromatography. Zone electrophoresis is performed to ensure that only a gamma-mobility component is present. BufferSystem: Glycine buffered saline, pH 7.4 containing 0.099% Sodium azide, 0.1% EACA, 0.01% Benzamidine, 1mM EDTA |
| Applications | Suitable for use in a variety of gel techniques including RID, double diffusion, IEP, and rocket IEP. The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
RID & Rocket RID: 5µl antiserum/cm2 in gel vs 5µl IgE myeloma (1mg/ml) Neat - 1:100 Double Diffusion: 10µl antiserum vs 1µl myeloma sera (1mg/ml) IEP: 100µl antiserum vs 5µl IgE myeloma sera (1mg/ml) |
| Specificity | This antidody reacts to IgE. Gives a single arc when tested by IEP against human IgE myeloma sera. No arcs are visible when tested by IEP against normal human serum. Identity has been confirmed by double diffusion (Ouchterlony) against human IgE and a known anti-human IgE. Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C.
DO NOT FREEZE! Shelf life: one year from despatch. |
7 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1456B | Sheep IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1456F | Sheep IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1456T | Sheep IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1456TR | Sheep IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 305 | |||||
| R1456HRP | Sheep IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 325 | |||||
| R1456AP | Sheep IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 345 | |||||
| R1433R | Sheep IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 465 | |||||
Click here to see all secondary antibodies for 'AP00816PU-N IgE'.