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Formalin-fixed, paraffin-embedded human breast carcinoma stained with Catenin beta antibody (Cat. AP00607) using peroxidase-conjugate and AEC. Note membrane staining of tumor cells.

 

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AP00607PU-N  Catenin beta antibody

see related secondary antibodies
see all 65 Catenin beta products
0.1 mg / no delivery possible
please contact the manufacturer

Quick Overview

Rabbit anti Catenin beta -

Synonyms

Beta-catenin, Catenin beta-1, CTNNB1, CTNNB, ,

Product review
Please read our product review about Catenin beta: Antibodies to Catenins.

Product Description

Rabbit anti Catenin beta -, Presentation: Aff - Purified. Product is tested for Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsImmunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu ), Mouse ( Ms )
PresentationAff - Purified
HostRabbit
Catalog NumberAP00607PU-N
Swiss Prot. No.P35222
Quantity0.1 mg
Priceno delivery possible
DeliveryNot USA/Canada
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AP00607PU-N.pdf

Datasheet Extract

Polyclonal Antibody to β-Catenin
Concentration0.2 mg/ml
BackgroundThe catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. β-catenin also binds to N-cadherin and coimmunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between α-actinin and α-catenin. Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.
ImmunogenA synthetic peptide from the C-terminus of the β-catenin protein.
Swiss Num.: P35222
Format
State: Liquid purified IgG
Purification: Immunogen affinity chromatography
BufferSystem: 10mM PBS, pH 7.6, with 0.2% BSA and 15mM sodium azide.
ApplicationsWestern Blotting: Ab 2 µg/ml for 2 hours at RT. Immunoprecipitation. Immunohistology (Formalin/paraffin): Ab 1:250-1:500 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive Control: MCF-7 cells. Breast carcinoma.
SpecificityThis antibody is excellent for staining of beta Catenin in formalin-fixed, paraffin-embedded tissues. Molecular weight of antigen: 92kDa. Cellular Localization: Cytoplasmic and cell membrane.
Species: Human and mouse.
StorageStore at 2-8°C or in aliquots at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
PicturesFormalin-fixed, paraffin-embedded human breast carcinoma stained with Catenin beta antibody (Cat. AP00607) using peroxidase-conjugate and AEC. Note membrane staining of tumor cells.

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'AP00607PU-N Catenin beta'.