AP00582PU-N Estrogen Receptor alpha / ESR1 (N-term) antibody
see related secondary antibodiessee all 119 Estrogen Receptor alpha / ESR1 products
0.5 mg / no delivery possible
please contact the manufacturer
Quick Overview
Rabbit anti Estrogen Receptor alpha / ESR1
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Rabbit anti Estrogen Receptor alpha / ESR1 , Presentation: Purified. Product is tested for Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Hamster ( Hst ), Human ( Hu ), Mouse ( Ms ), Por ( Por ), Rabbit ( Rb ), Rat ( Rt ), Sheep ( Sh ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00582PU-N |
| Swiss Prot. No. | P03372 |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00582PU-N.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. |
| Immunogen | A synthetic peptide from the N-terminus of human estrogen receptor, alpha protein. Swiss Num.: P03372 |
| Format | State: Liquid purified IgG Purification: Protein A chromatography. BufferSystem: 10mM PBS, pH 7.4, with 0.2% BSA & 0.09% sodium azide. |
| Applications | Immunoprecipitation; (Denatured): Use Protein A and Ab at 10 µg/mg protein lysate). Western Blotting: 2.5-5µg/ml for 2hrs at RT. Immunohistology (formalin/paraffin): use Ab at 10-20µg/ml for 30 min at RT. (Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0) for 10-20 min followed by cooling at RT for 20 min.) Positive Control: MCF-7 Cells or Breast carcinoma. |
| Specificity | Highly specific for Estrogen receptor alpha. Molecular weight of antigen: 67kDa. Cellular Localization: Nuclear. Species: Human, Rat, Mouse, Rabbit, Sheep, Pig, and Cow. |
| Storage | Store at 2-8°C or in aliquots at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Al Saati, et al. (1993) Int J Cancer, 55:651-654.
2. Abbondanza C et. al. Steroids, 1993, 58:4-12. |
| Pictures | Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER alpha Ab (Cat. AP00528) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP00582PU-N Estrogen Receptor alpha / ESR1 '.
