AP00581PU-N Estrogen Receptor alpha / ESR1 (C-term) antibody
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0.5 mg / no delivery possible
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Quick Overview
Rabbit anti Estrogen Receptor alpha / ESR1
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Rabbit anti Estrogen Receptor alpha / ESR1 , Presentation: Purified. Product is tested for Immunoprecipitation ( IP ), Paraffin Sections ( P )
Properties
| Applications | Immunoprecipitation ( IP ), Paraffin Sections ( P ) |
| Reactivity | Hamster ( Hst ), Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00581PU-N |
| Swiss Prot. No. | P03372 |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00581PU-N.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. |
| Immunogen | A synthetic peptide derived from the C-terminus of human estrogen receptor, alpha protein. Swiss Num.: P03372 Remarks: Mol. Wt. of Antigen: 67 kDa |
| Format | State: Liquid IgG Purification: Protein A chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide. |
| Applications | Gel Supershift (not verified).
Immunoprecipitation (Denatured verified); Use Protein A, Ab 10 µg/mg protein lysate. Western Blotting: 2.5-5 µg/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): Use Ab at 1-2 µg/ml for 30 min at RT. (Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.) Positive Control: MCF-7 cells. Breast carcinoma. |
| Specificity | This antibody detects Estrogen Receptor.
Cellular Localization: Nuclear. Species: Human, Mouse and Hamster. Does not react with rat. |
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Al Saati, et al. (1993) Int J Cancer, 55:651-654.
2. Abbondanza C et. al. Steroids, 1993, 58:4-12. |
| Pictures | Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER,alpha (AP00581PU-N ) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP00581PU-N Estrogen Receptor alpha / ESR1 '.
