AP00580PU-N Estrogen Receptor alpha / ESR1 antibody
see related secondary antibodiessee all 119 Estrogen Receptor alpha / ESR1 products
0.1 mg / no delivery possible
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Quick Overview
Rabbit anti Estrogen Receptor alpha / ESR1
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Rabbit anti Estrogen Receptor alpha / ESR1 , Presentation: Aff - Purified. Product is tested for Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Hamster ( Hst ), Human ( Hu ), Mouse ( Ms ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | AP00580PU-N |
| Swiss Prot. No. | P03372 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00580PU-N.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy. |
| Immunogen | Synthetic peptide derived from C-terminal of human estrogen receptor. Swiss Num.: P03372 |
| Format | State: Liquid purified IgG Purification: Immunogen affinity chromatography BufferSystem: 10mM PBS, pH 7.6, with 0.2% BSA and 15mM sodium azide. |
| Applications | Western Blotting. Immunohistology (Formalin/paraffin): 1:100 - 1:200 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive Control: MCF-7 Cells, Breast carcinomas, Colon carcinomas. |
| Specificity | Recognizes a protein named estrogen receptor (ER) alpha. This antibody strongly stains the nucleus of epithelial
cells in breast carcinomas and is excellent for staining of ER in formalin-fixed, paraffin-embedded tissues. Molecular weight of antigen: 67kDa. Species: Human, Mouse and Hamster. Does not react with rat. |
| Storage | Store at 2-8°C or in aliquots at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Pictures | Formalin-fixed. Paraffin-embedded human breast carcinoma stained with estrogen receptor (Cat.# AP00580) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP00580PU-N Estrogen Receptor alpha / ESR1 '.
