AP00558PU-N Cullin-3 (CUL-3) antibody
see related secondary antibodiessee all 10 Cullin-3 products
0.5 mg / no delivery possible
please contact the manufacturer
Quick Overview
Rabbit anti Cullin-3 (CUL-3) -
Synonyms
DP3036 Cullin 3 CUL3
Product review
Please read our product review about Cullin-3 (CUL-3): Antibodies to Cullin Protein Family.
Product Description
Rabbit anti Cullin-3 (CUL-3) -, Presentation: Purified. Product is tested for Paraffin Sections ( P )
Properties
| Applications | Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00558PU-N |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00558PU-N.pdf |
Datasheet Extract
| Polyclonal Antibody to Cullin-3 (CUL-3) | |
| Concentration | 1.0 mg/ml |
| Background | CUL-3 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b, and -5), homologous to the S. cerevisiae cdc53 gene. Cullin-3 is proposed to be a part of E3 protein-ubiqutin ligase. It forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by protesomes. Cyclin E is an activator of cdk2, which leads the cell to S phase. Cul-3-/- mice show increased accumulation of Cyclin E protein and disruption of normal cell cycle regulation in vivo. Thus Cullin-3 is important in the regulation of S phase of cell cycle. |
| Immunogen | A synthetic peptide from the N-terminus of human Cullin-3 protein. Remarks: Mol. Wt. of Antigen: 85 kDa |
| Format | State: Liquid IgG Purification: Protein A chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide. |
| Applications | Immunohistology (Formalin/paraffin); use Ab at 2-4 µg/ml for 30 min at RT; staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control: Tonsil. |
| Specificity | This antibody detects Cullin-3.
Cellular Localization: Nuclear. Species: Human, Mouse. |
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Kipreos ET, et. al. Cell 85:829-839, 1996. |
| Pictures | Formalin-fixed, paraffin-embedded human tonsil stained with Cullin-3 (AP00558PU-N) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 170,00 € | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160,00 € | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160,00 € | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / 170,00 € | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170,00 € | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 200,00 € | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'AP00558PU-N Cullin-3 (CUL-3)'.
