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Formalin-fixed, paraffin-embedded human tonsil stained with Ki67 (AP00538PU-N) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.

 

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AP00538PU-N  Ki-67 antibody

see related secondary antibodies
see all 36 Ki-67 products
0.5 mg / no delivery possible
please contact the manufacturer

Quick Overview

Rabbit anti Ki-67

Synonyms

Antigen Ki67, MKI67, Proliferation Marker, , antigen identified by monoclonal antibody Ki-67, KIA, Proliferation related Ki 67 antigen

Product review
Please read our product review about Ki-67: Proliferation Marker Antibodies.

Product Description

Rabbit anti Ki-67 , Presentation: Purified. Product is tested for Paraffin Sections ( P )

Properties

ApplicationsParaffin Sections ( P )
ReactivityHuman ( Hu ), Rat ( Rt )
PresentationPurified
HostRabbit
Catalog NumberAP00538PU-N
Swiss Prot. No.P46013
Quantity0.5 mg
Priceno delivery possible
DeliveryNot USA/Canada
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AP00538PU-N.pdf

Datasheet Extract

Concentration1 mg/ml
BackgroundThe Ki67 is a nuclear protein, which is expressed in the proliferating cells. Ki67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiscent) phase are negative for this protein.
ImmunogenA synthetic peptide from the human Ki67 protein
Swiss Num.: P46013
Format
State: Liquid antiserum
Purification: Protein A chromatography
BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
ApplicationsImmunohistology (formalin/paraffin): Use Ab at 10-20 µg/ml for 30 min at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: Tonsil or Breast Carcinoma.
SpecificityThis antibody detects Ki67.
Cellular Localization: Nuclear.
Species: Human, Rat.
StorageStore the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Gerdes J, et al. Am J Pathol 1991; 138:867-73
2. Key G, et al. J Clin Pathol 1993;46:1080-4.
PicturesFormalin-fixed, paraffin-embedded human tonsil stained with Ki67 (AP00538PU-N) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 305
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 295
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 295
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 305
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 305
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 335
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 455
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 455
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 455
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 455
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 455
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 465
   

Click here to see all secondary antibodies for 'AP00538PU-N Ki-67'.