AP00524PU-N Focal Adhesion Kinase / FAK antibody
see related secondary antibodiessee all 62 Focal Adhesion Kinase / FAK products
0.5 mg / no delivery possible
please contact the manufacturer
Quick Overview
Rabbit anti Focal Adhesion Kinase / FAK -
Synonyms
Protein-tyrosine Kinase 2 FADK1 FAK1 PTK2 PP125FAK
Product review
Please read our product review about Focal Adhesion Kinase / FAK: Antibodies to Focal Adhesion Kinase (FAK).
Product Description
Rabbit anti Focal Adhesion Kinase / FAK -, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Canine ( Can ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ), Frog ( Frog ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00524PU-N |
| Swiss Prot. No. | Q05397 |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00524PU-N.pdf |
Datasheet Extract
| Polyclonal Antibody to FAK//(Focal Adhesion Kinase) | |
| Concentration | 1 mg/ml |
| Background | FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.
|
| Immunogen | Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK. Swiss Num.: Q05397 |
| Format | State: Liquid Purification: Protein A affinity purified immunoglobulin fraction BufferSystem: 10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer. |
| Applications | Immunofluorescence. Western Blotting: Ab 5-10 µg/ml for 2hrs at RT. Recommended positive control: HUVEC or IMR-5 Cells. |
| Specificity | Reacts with FAK from severals species. Cellular Localization: Cytoplasmic, Focal Contacts. Mol. Wt. of Antigen: 125kD. Species: Human, Mouse, Rat, Dog, and Frog. |
| Storage | Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch. |
| References | 1. Hanks SK; Polte TR. Bioessays, 1997, 19(2):137-45.
2. Ilic D; et al. Journal of Cell Science, 1997, 110:401-7. 3. Owens LV; et al. Cancer Research, 1995, 55(13):2752-5. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP00524PU-N Focal Adhesion Kinase / FAK'.
