AP00499PU-N Cullin-2 (CUL-2) antibody
see related secondary antibodiessee all 8 Cullin-2 products
0.5 mg / no delivery possible
please contact the manufacturer
Quick Overview
Rabbit anti Cullin-2 (CUL-2) -
Synonyms
DP3035 Cullin 2 CUL2
Product review
Please read our product review about Cullin-2 (CUL-2): Antibodies to Cullin Protein Family.
Product Description
Rabbit anti Cullin-2 (CUL-2) -, Presentation: Purified. Product is tested for Paraffin Sections ( P ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Paraffin Sections ( P ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00499PU-N |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00499PU-N.pdf |
Datasheet Extract
| Polyclonal Antibody to Cullin-2 (CUL-2) | |
| Concentration | 1 mg/ml |
| Background | CUL-2 is a member of the family of human cullin genes (CUL-1, -2, -3,-4a,-4b, and -5), homologous to the S. cerevisiae cdc53 gene. CUL-1 forms a complex with human p19skp1 and F box protein p45skp2. Together with cdc53 (known as CULA), as three subunits, they form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. |
| Immunogen | A peptide corresponding to amino acid residue 733-745 (Cys-RSQASADEYSYVA) at C-terminus of the human Cullin-2 |
| Format | State: Liquid Purification: Protein A affinity purified immunoglobulin fraction BufferSystem: 10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer. |
| Applications | Western Blotting: Ab 5-10µg/ml for 2hrs at RT. Immunohistology (Formalin/paraffin): Ab 1:25- 1:50 for 30 min. RT.Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: MAD109 Cells, Tonsil. |
| Specificity | Highly specific for Cullin 2. Does not react with other Cullins. Cellular Localization: Nuclear. Mol. Wt. of Antigen: 76kD. Species: Human and Mouse. |
| Storage | Store at 4°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch. |
| References | 1. Kipreos ET, et. al. Cell 85:829-839, 2.
2. Pagano M. FASEB J. 11:1067-1075, 1997. 3. Pause A, et. al. Proc. Natl.Acad.Sci.USA. 94:2156-2161, 1997. |
| Pictures | Formalin-fixed, paraffin-embedded human tonsil stained with Cullin-2 Ab-1 (Cat. AP00499) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 170,00 € | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160,00 € | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160,00 € | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / 170,00 € | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170,00 € | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 200,00 € | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'AP00499PU-N Cullin-2 (CUL-2)'.
