AP00497PU-N Cullin-1 (CUL-1) antibody
see related secondary antibodiessee all 8 Cullin-1 products
0.5 mg / no delivery possible
please contact the manufacturer
Quick Overview
Rabbit anti Cullin-1 (CUL-1) -
Synonyms
DP3034 Cullin 1 CUL1
Product review
Please read our product review about Cullin-1 (CUL-1): Antibodies to Cullin Protein Family.
Product Description
Rabbit anti Cullin-1 (CUL-1) -, Presentation: Purified. Product is tested for Paraffin Sections ( P ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Paraffin Sections ( P ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | AP00497PU-N |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AP00497PU-N.pdf |
Datasheet Extract
| Polyclonal Antibody to Cullin-1 (CUL-1) | |
| Concentration | 1 mg/ml |
| Background | CUL-1 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a,-4b, and -5) homologous to the S. cerevisiae cdc53 gene. CUL 1 forms a complex with human p19skp1 and F box protein p45skp2, both of which, together with cdc53 (known as CULA), as three subunits, form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. |
| Immunogen | A peptide from the C-terminus of the human Cullin-1. |
| Format | State: Liquid Purification: Protein A affinity purified immunoglobulin fraction BufferSystem: 10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer. |
| Applications | Immunoprecipitation (Denaturated)(Use Protein A): Ab at 10 micrograms/mg protein lysate. Western Blotting: Ab 5-10 micrograms/ml for 2hrs at RT. Immunohistology (Formalin/paraffin): Ab 5-10 micrograms/ml for 30 min at RT (Staining of formalin-fixed tissues REQUIRES boiling
tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.) Recommended positive control: HeLa Cells, Tonsil. |
| Specificity | Highly specific for Cullin 1. Does not react with other Cullins. Cellular Localization: Nuclear. Mol. Wt. of Antigen: 86kD. Species: Human and Mouse. Does not react with Rat. |
| Storage | Store at 4°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch. |
| References | 1. Kipreos ET, et. al. Cell 85:829-839, 2.
2. Pagano M. FASEB J. 11:1067-1075, 3. 3. Michel JJ, et. al. Cell Growth & Differentiation 9:435-449, 1998. |
| Pictures | Formalin-fixed, paraffin-embedded human tonsil stained with Cullin-1 Ab (Cat. AP00497) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AP00497PU-N Cullin-1 (CUL-1)'.
