AM03021SU-N PLSCR1 antibody
see related secondary antibodiessee all 10 PLSCR1 products
0.1 ml / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti PLSCR1 13A6 [TEC-23]
Synonyms
Phospholipid Scramblase 1,
Product Description
Mouse anti PLSCR1 13A6 [TEC-23], Presentation: Ascites
Properties
| Reactivity | Rat ( Rt ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | 13A6 [TEC-23] |
| Catalog Number | AM03021SU-N |
| Quantity | 0.1 ml |
| Price | US$ 315 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM03021SU-N.pdf |
Datasheet Extract
| Monoclonal Antibody to PLSCR1 | |
| Background | PLSCR1 (Phospholipid Scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction.
Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors. |
| Immunogen | BALB/c mice were immunized with pooled lipid rafts isolated from RBL-2H3 cells.
Hybridoma cells were obtained after fusion of SP02 mouse myeloma cells with spleen cells of immunized mice using standard procedures. |
| Format | State: Liquid undiluted ascites with 15 mM sodium azide as preservative. |
| Applications | Immunoprecipitation.
Western Blotting: 1 μg/ml. Flow Cytometry. Immunocytochemistry. |
| Specificity | This antibody binds to rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa membrane-associated protein accelerating bidirectional movement of plasma membrane phospholipids during conditions of elevated calcium. Species: Rat. |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Pastorelli C, Veiga J, Charles N, Voignier E, Moussu H, Monteiro RC, Benhamou M: IgE
receptor type I-dependent tyrosine phosphorylation of phospholipid scramblase. J Biol Chem. 2001 Jun 8;276(23):20407-12. Epub 2001 Mar 20. 2. Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ: Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. Cancer Res. 2002 Jan 15;62(2):397-402. 3. Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH: Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J Virol. 2004 Sep;78(17):8983-93. 4. Smrz D, Lebduska P, Dráberová L, Korb J, Dráber P: Engagement of Phospholipid Scramblase 1 in Activated Cells: IMPLICATION FOR PHOSPHATIDYLSERINE EXTERNALIZATION AND EXOCYTOSIS. J Biol Chem. 2008 Apr 18;283(16):10904-18. |
| Protocols | Western Blot:
Positive control: RBL rat basophilic leukemia cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% Triton X-100 in 20 mM Tris/Cl, 100 mM NaCl, 2 mM EDTA, 2 mM Na3Vo4, 10 mM glycerolphosphate, pH 8.0, 1 mM PMSF, 10 μg/ml aprotinin). Incubate 20 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min. Note: Both reducing and non-reducing conditions |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM03021SU-N PLSCR1'.