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AM00629PU-N  DNA PKcs antibody

see related secondary antibodies
see all 29 DNA PKcs products
0.1 mg / no delivery possible
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Quick Overview

Mouse anti DNA PKcs 42-26

Synonyms

DNA-PKcs PRKDC HYRC XRCC7

Product review
Please read our product review about DNA PKcs: Antibodies to XRCC Proteins.

Product Description

Mouse anti DNA PKcs 42-26, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Paraffin Sections ( P )

Properties

ApplicationsImmunofluorescence ( IF ), Paraffin Sections ( P )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
Clone42-26
Catalog NumberAM00629PU-N
Quantity0.1 mg
Priceno delivery possible
DeliveryNot USA/Canada
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AM00629PU-N.pdf

Datasheet Extract

Monoclonal Antibody to DNA-PKcs//(DNA-dependent Protein Kinase)
Concentration0.2 mg/ml
BackgroundDNA-PKcs phosphorylates certain transcription factors, including Sp 1, Oct-1, p53, and SV40 large T antigen in vitro.
In vivo, it is involved in reparing double strand DNA breaks.
ImmunogenHuman DNA-dependent Protein Kinase purified from HeLa cells (see ref. 1)
Format
State: Liquid purified IgG fraction
Purification: Protein G chomatography
BufferSystem: 10 mM PBS, pH 7.4, containing 0.2% BSA and 0.09% sodium azide
ApplicationsImmunofluorescence.
Immunohistochemistry on formalin-fixed paraffin embedded sections: 1/400, 20 min at RT. (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, and use of a high-sensitivity detection system).
Recommended positive control: Testis.
SpecificityThe antibody recognizes the intact DNA-PKcs as well as its degraded products.
Cellular localization: Nuclear.
Molecular Weight of Antigen: 460 kDa.
Epitope: aa 2713-3198.
Species: Human.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Carter T. et al. Mol Cell Biol, 1990, 10:6460-71.
2. Han Z. et al. J Biol Chem, 1996, 271:25035-40.
3. Kirchgessner CU. et al. Science, 1995 Feb 24, 267(5201): 1178-83.
4. Poltoratsky VP. et al. J Immunol, 1995, 155(10): 4529-33.
5. Konno-Sato S. et al. Biochem Mol Biol Intl, 1993, 31:113-24.

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / 180,00 €
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / 160,00 €
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / 160,00 €
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / 170,00 €
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / 190,00 €
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / 200,00 €
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / 320,00 €
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / 320,00 €
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / 320,00 €
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / 320,00 €
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / 320,00 €
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / 330,00 €
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / 330,00 €
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / 330,00 €
   

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