AM00558PU-N Ep-CAM / TACSTD1 / CD326
see related secondary antibodiessee all 25 Ep-CAM / TACSTD1 / CD326 products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti Ep-CAM / TACSTD1 / CD326 323/A3
Synonyms
EPCAM EGP KSA GA733-2 M1S2 M4S1 MIC18 TROP1 FocusOn125 Epithelial cell adhesion molecule, Epithelial cell surface antigen
Product Description
Mouse anti Ep-CAM / TACSTD1 / CD326 323/A3, Presentation: Purified. Product is tested for Flow cytometry ( F ), Immunofluorescence ( IF ), Paraffin Sections ( P )
Properties
| Applications | Flow cytometry ( F ), Immunofluorescence ( IF ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 323/A3 |
| Catalog Number | AM00558PU-N |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00558PU-N.pdf |
Datasheet Extract
| Monoclonal Antibody//to Epithelial Specific Antigen (ESA /Ep-CAM) | |
| Alternate names | CD326, KSA, Lymphocyte antigen 74, M1S2, M4S1, GA733-2, MIC18 |
| Concentration | 0.2 mg/ml |
| Background | EGP40 is a 40 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. It reportedly distinguishes adenocarcinomas from pleural mesotheliomas. |
| Immunogen | MCF-7 human breast cancer cells (see Ref. 1). |
| Format | State: Liquid purified Ig fraction Purification: Protein G chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide |
| Applications | Flow Cytometry.
Immunofluorescence. Radioimaging. Immunohistology (Formalin/paraffin): 1-2 µg/ml for 30 min at RT, staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1 mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37 °C. Positive Control: Breast carcinoma. |
| Specificity | This antibody detects Epithelial Specific Antigen. Mol. Wt. of Antigen: 40-43 kDa. Cellular Localization: Cell membrane. Species: Human. |
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Edwards DP, et. al. Cancer Res, 1986, 46:1306-17. |
| Pictures | Formalin-fixed, paraffin-embedded human breast carcinoma stained with ESA (AM00558PU-N) using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
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