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Formalin-fixed, paraffin-embedded human breast carcinoma stained with Progesterone Receptor (AM00539PU-N) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.

 

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AM00539PU-N  Progesterone Receptor antibody

see related secondary antibodies
see all 76 Progesterone Receptor products
0.1 mg / no delivery possible
please contact the manufacturer

Quick Overview

Mouse anti Progesterone Receptor hPRa5

Synonyms

PR, PGR, NR3C3, , Nuclear receptor subfamily 3 group C member 3

Product review
Please read our product review about Progesterone Receptor : Antibodies to Progesterone Receptor.

Product Description

Mouse anti Progesterone Receptor hPRa5, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsImmunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityGenua Pig ( GP ), Human ( Hu ), Monkey ( Mky ), Mouse ( Ms ), Rat ( Rt )
PresentationPurified
HostMouse
IsotypeIgG1
ClonehPRa5
Catalog NumberAM00539PU-N
Swiss Prot. No.P06401
Quantity0.1 mg
Priceno delivery possible
DeliveryNot USA/Canada
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AM00539PU-N.pdf

Datasheet Extract

Concentration0.2 mg/ml
BackgroundHuman PgR exists in two forms: 116 kDa (B-form) and 81 kDa (A-form). It acts as ligand activated transcription factor to regulate expression of the target genes. Null mutation in PgR gene leads to pleiotrophic reproductive abnormalities.
ImmunogenPartially purified PgR from a human endometrial carcinoma (EnCa 101) grown in athymic mice.
Swiss Num.: P06401
Remarks: Mol. Wt. of Antigen: 116 kDa (triplet, B-form) and 81 kDa (singlet, A-form)
Epitope: N-terminal half of human progesterone
Format
State: Liquid ascites fluid
Purification: Protein G chromatography
BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
ApplicationsImmunofluorescence.
Immunoprecipitation (Native only): use Protein G; 2 µg/mg protein lysate.
Sedimentation in Sucrose Gradient (1).
Western Blotting: 1-2 µg/ml for 2 hrs at RT.
Immunohistology (Formalin/paraffin): recommended for murine tissues only, 2-4 µg/ml for 30 min at RT; staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control: T47D cells or breast carcinoma.
SpecificityThis antibody detects Progesterone Receptor.
Species: Human, Baboon, Monkey, Mouse, Rat, Guinea pig.
Add. InformationCellular Localization: Nuclear
StorageStore the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Clarke CL, et. al. Endocrinology, 1987, 121(3):1123-32.
PicturesFormalin-fixed, paraffin-embedded human breast carcinoma stained with Progesterone Receptor (AM00539PU-N) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.


14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

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