AM00372PU-N Estrogen Receptor alpha / ESR1 antibody
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0.1 mg / no delivery possible
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Quick Overview
Rat anti Estrogen Receptor alpha / ESR1 D75
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Rat anti Estrogen Receptor alpha / ESR1 D75, Presentation: Purified. Product is tested for Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Monkey ( Mky ) |
| Presentation | Purified |
| Host | Rat |
| Isotype | IgG1 |
| Clone | D75 |
| Catalog Number | AM00372PU-N |
| Swiss Prot. No. | P03372 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00372PU-N.pdf |
Datasheet Extract
| Background | ER protein exhibits six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. It is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors may improve response to anti-estrogen therapy. |
| Immunogen | Estrogen receptor purified from MCF-7 cells. Swiss Num.: P03372 Remarks: Mol. Wt. of Antigen: 67 kDa |
| Format | State: Liquid ascites Purification: Protein G chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide. |
| Applications | Western Blotting: 1-2 µg/ml for 2hrs at RT.
Immunohistology (formalin/paraffin): use 2-4µg/ml for 30 min at RT, (Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.). Positive Control: MCF-7 cells. Breast Carcinoma. |
| Specificity | This antibody detects Estrogen Rceptor. Species: Human, Baboon, Chimoazee, Monkey. |
| Add. Information | Cellular Localization: Nuclear.
Does not react with rat. |
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Green G, et al. (1984) J. Steroid Biochem. 20: 51-56.
2. Katzenellenbogen B, et al. (1987) Biochemistry 26: 2364-2373. 3. Miller R T, Hapke M R, Greene G L. (1993) Cancer 71: 3541-3546. |
| Pictures | Formalin-fixed, paraffin-embedded human breast carcinoma stained with AM00372PU-N using peroxidase-conjugate and AEC chromogen. Note intense nuclear staining of tumor cells. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
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