AM00370AF-N Estrogen Receptor alpha / ESR1 antibody
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0.1 mg / no delivery possible
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Quick Overview
Mouse anti Estrogen Receptor alpha / ESR1 TE111.5D11
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Mouse anti Estrogen Receptor alpha / ESR1 TE111.5D11, Presentation: Azide Free. Product is tested for Electrophoretic Mobility Shift Assay ( EMSA ), Functional assay ( FN ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Electrophoretic Mobility Shift Assay ( EMSA ), Functional assay ( FN ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Canine ( Can ), Chicken ( Chk ), Hamster ( Hst ), Human ( Hu ), Mouse ( Ms ), Por ( Por ), Rabbit ( Rb ), Rat ( Rt ), Sheep ( Sh ) |
| Presentation | Azide Free |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | TE111.5D11 |
| Catalog Number | AM00370AF-N |
| Swiss Prot. No. | P03372 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00370AF-N.pdf |
Datasheet Extract
| Concentration | 1 mg/ml |
| Background | ER gene consists of more than 140 kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. |
| Immunogen | 35 kDa, C-terminal fragment of human ER expressed in E. coli Swiss Num.: P03372 AA Sequence: 302-553 Remarks: Mol. Wt. of Antigen: 67 kDa |
| Format | State: Liquid ascites fluid Purification: Protein G chromatography. BufferSystem: 10 mM PBS, pH 7.4 |
| Applications | Immunoprecipitation (Native and denatured): use Protein G, 2µg/mg protein lysate.
Western Blotting: 1-2 µg/ml for 2hrs at RT. Blocks binding of estrogen to ER. Chromatin Precipitation. Positive Control: T47D cells. |
| Specificity | This antibody detects Estrogen Receptor. Species: Human, Cow, Pig, Dog, Mouse, Rat, Sheep, Rabbit, Hamster, and Chicken. |
| Add. Information | Cellular Localization: Nuclear. |
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Abbondanza C et. al. Steroids, 1993, 58:4-12. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00370AF-N Estrogen Receptor alpha / ESR1 '.
