AM00306PU-N p35 Nck5a antibody
see related secondary antibodiessee all 4 p35 Nck5a products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti p35 Nck5a ES19
Synonyms
DM3163,
Product Description
Mouse anti p35 Nck5a ES19, Presentation: Purified. Product is tested for Immunoprecipitation ( IP )
Properties
| Applications | Immunoprecipitation ( IP ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | ES19 |
| Catalog Number | AM00306PU-N |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00306PU-N.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | p35nck5a, is a neuron-specific activator of Cdk5. Treatment of cultured neurons with cyclohexamide induces the rapid disappearance of p35 accompanied by parallel inactivation of the kinase activity of Cdk5. The disappearance of p35 is blocked with proteasome inhibitors, indicating the involvement of proteasome. The degradation of p35 is nduced with okadaic acid in the presence of ATP in neuron extracts. The degradation of p35 by proteasome in cultured neurons is stimulated by okadaic acid in the absence of cyclohexamide. Cdk5 and its neuron-specific regulator p35 are essential for neuronal migration and for the laminar configuration of the cerebral cortex. p35/Cdk5 kinase concentrates at the leading edges of axonal growth cones and regulates neurite outgrowth in cortical neurons in culture. p35/Cdk5 kinase co-localizes with Rac in neuronal growth cones. |
| Immunogen | Recombinant Human p35 protein. Remarks: Mol. Wt. of Antigen: 35 kDa |
| Format | State: Liquid ascites fluid Purification: Protein A chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide. |
| Applications | Immunoprecipitation: Co-precipitates cdk5; Use Protein A; Ab 2µg/mg protein lysate.
Kinase Assay (Not verified). Recommended positive control: IMR-5 Cells. |
| Specificity | This antibody detects p35nck5a.
Cellular Localization: Cytoplasmic. Species: Human. |
| Storage | Store the antibody at 2 - 8 °C for one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Saito T, et al. Biochem Biophys Res Commun 1998 Nov 27;252(3):775-778.
2. Matsushita M, et al. Brain Res 1996 Sep 23;734(1-2):319-322. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00306PU-N p35 Nck5a'.
