AM00305PU-N E2F1 Transcription Factor antibody
see related secondary antibodiessee all 43 E2F1 Transcription Factor products
0.1 mg / no delivery possible
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Quick Overview
Mouse anti E2F1 Transcription Factor KH20
Synonyms
E2F-1, RBBP3, RBBP-3, PBR3, RBAP-1, RBAP1, , Retinoblastoma-binding protein 3, PRB-binding protein E2F-1, Retinoblastoma-associated protein 1
Product review
Please read our product review about E2F1 Transcription Factor : Antibodies to E2F Transcription Factor Family.
Product Description
Mouse anti E2F1 Transcription Factor KH20, Presentation: Purified. Product is tested for Electrophoretic Mobility Shift Assay ( EMSA ), Immunofluorescence ( IF )
Properties
| Applications | Electrophoretic Mobility Shift Assay ( EMSA ), Immunofluorescence ( IF ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | KH20 |
| Catalog Number | AM00305PU-N |
| Swiss Prot. No. | Q01094 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00305PU-N.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | E2F's are DNA-binding proteins that associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 also requires DP-1 for efficient DNA-binding and transcription modification. E2F1 is proposed to be involved in several cellular processes that range from tumor suppression, cell cycle progression, and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis. These observations place E2F among a group of apoptosis inducers that includes; c-Myc, Adenovirus E1A, and the human papilloma virus E7 protein. |
| Immunogen | Recombinant Human E2F-1 protein. Swiss Num.: Q01094 AA Sequence: 1-89 Remarks: Mol. Wt. of Antigen: ~60 kDa |
| Format | State: Liquid ascites fluid. Purification: Protein A chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide. |
| Applications | Gel Supershift: use Ab at 1mg/ml.
Immunofluorescence. Recommended positive control: MAD109 and HL-60 Cells. Not suitable for Immunohistology, Immunoprecipitation and Western Blotting. |
| Specificity | Thus antibody detects E2F-1 Transcription Factor.
Cellular Localization: Nuclear. Species: Human, Mouse, Rat. |
| Storage | Store the antibody at 2 - 8 °C for one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Helin K, et. al. J. Virology, 1994;68:5027-5035.
2. Saito M, et. al. Genomics, 1995;25:130-138. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00305PU-N E2F1 Transcription Factor '.
