AM00301PU-N p18INK4c antibody
see related secondary antibodiessee all 8 p18INK4c products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti p18INK4c DCS-118
Synonyms
DM3162, p18-INK6, CDKN2C, CDKN6,
Product Description
Mouse anti p18INK4c DCS-118, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | DCS-118 |
| Catalog Number | AM00301PU-N |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00301PU-N.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | p18eINK4c is a specific inhibitor of cdk4/cdk6 and is expressed during G1 -to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase. |
| Immunogen | Purified recombinant p18 INK4c protein. Remarks: Mol. Wt. of Antigen: 18 kDa |
| Format | State: Liquid ascites fluid Purification: Protein G chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide. |
| Applications | Immunofluorescence.
Immunoprecipitation: Co-precipitates cdk4; Use Protein G; Ab 1-2µg/mg protein lysate. Western Blotting: Ab 1-2µg/ml for 2hrs at RT. Immunohistology (Formalin/paraffin): Use Ab at 2-4µg/ml for 30 min at RT. Staining of formalin/paraffin tissues is requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: HeLa Cells, Tonsil. |
| Specificity | This antibody detects p18ink4c.
Cellular Localization: Nuclear. Species: Human, mouse. |
| Storage | Store the antibody at 2 â?? 8 °C for one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Khleif SN; et al. Proc Natl Acad Sci USA, 1996 93(9):4350-4.
2. Lapointe J; et al. Cancer Res, 1996, 56:4586-9. 3. Palmero I; Peters G. Cancer Surv, 1996, 27:351-67. |
| Pictures | Formalin-fixed, paraffin-embedded human tonsil stained with p18 (AM00301PU-N) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00301PU-N p18INK4c'.
