AM00286PU-N Tubulin alpha antibody
see related secondary antibodiessee all 55 Tubulin alpha products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti Tubulin alpha DM1A
Synonyms
Microtubule Marker, Loading Control, alpha-Tubulin, , ,
Product review
Please read our product review about Tubulin alpha: Antibodies to Loading Controls.
Product Description
Mouse anti Tubulin alpha DM1A, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | All Species ( All Species ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | DM1A |
| Catalog Number | AM00286PU-N |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00286PU-N.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: α and β. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. |
| Immunogen | Native Chick Brain Microtubules AA Sequence: 426-450 Remarks: Mol. Wt. of Antigen: 57 kDa |
| Format | State: Liquid purified Ig fraction Purification: Protein G chromatography BufferSystem: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide. |
| Applications | Electron Microscopy.
Immunofluorescence. Immunoprecipitation: Native and denatured; Use Protein G; Ab 2 micrograms/mg protein lysate. Western Blotting: 1-2 micrograms/ml for 2hrs at RT (1). Immunohistochemistry (Formalin/Paraffin): Use Ab at 1:400 for 30 min at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. Recommended positive control: Ls174T, MAD109 Cells, Skin, or Lung. |
| Specificity | The antibody causes the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by
70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Cellular Localization: Cytoplasmic Species: Human, Cow, Pig, Rat, Mouse, Guine pig, Gerbil, Frog, Chicken. |
| Storage | Store the antibody at 2 - 8 °C for one month or in aliquots at -20 °C for longer.
Shelf life: one year from despatch. |
| References | 1. Blose SH, et. al. J Cell Biol, 1984, 98:847-58.
2. Breitling F, et. al. J Mol Biol, 1986, 189:367-70. 3. Berry S, et. al. Biochim Biophys Acta, 1989, 1012:46-56. 4. Roychowdhury S, et. al. Biochemistry, 1993, 32:4955-61. |
| Pictures | Formalin-fixed, paraffin-embedded human lung stained with Tubulin-alpha Ab (AM00286PU-N) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of ciliated epithelium of bronchioles.
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00286PU-N Tubulin alpha'.
