AM00271PU-N DNA-repair protein complementing XP-A cells antibody
see related secondary antibodiessee all 9 DNA-repair protein complementing XP-A cells products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti DNA-repair protein complementing XP-A cells 12F5
Synonyms
XPA, XPAC, , Xeroderma pigmentosum group A-complementing protein
Product Description
Mouse anti DNA-repair protein complementing XP-A cells 12F5, Presentation: Purified. Product is tested for Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | 12F5 |
| Catalog Number | AM00271PU-N |
| Swiss Prot. No. | P23025 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00271PU-N.pdf |
Datasheet Extract
| Monoclonal Antibody to Human XPA | |
| Concentration | 0.2 mg/ml |
| Background | The XPA (xeroderma pigmentosum group A) protein specifically recognizes the UV-or chemically damaged DNA lesions, and triggers the nucleotide excision repair process. XPA binds to the replication protein A (RPA) or the excision repair cross complementing 1 protein (ERCC1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a > 2000 fold increased risk to develop skin cancer at sun-exposed areas. |
| Immunogen | Recombinant human XPA protein. Swiss Num.: P23025 Remarks: Mol. Wt. of Antigen: ~40kDa. |
| Applications | Western Blotting (Ab 1-2µg/ml for 2hrs at RT)
Immunohistology (Formalin/paraffin) (Use Ab at 2-4µg/ml for 30 minute at RT) [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] |
| Specificity | This antibody detects XPA. Species: Human. |
| Storage | Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer.
Shelf life: one year from despatch. |
| References | 1. Evans E, et al. (1997) EMBO J, 16:625-638.
2. Stoop H, et al. (2001) Lab Invest, 81:919-927. |
| Pictures | Formalin-fixed, paraffin-embedded human tonsil stained with AM00271PU-N using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||