AM00262PU-N p300 antibody
see related secondary antibodiessee all 28 p300 products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti p300 NM11
Synonyms
DM374P,
Product Description
Mouse anti p300 NM11, Presentation: Purified. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ), Mink ( Mink ), Monkey ( Mky ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | NM11 |
| Catalog Number | AM00262PU-N |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00262PU-N.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | p300 is a transcriptional adapter protein which is characterized by three cysteine- and histidine-rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300, and p300-associated proteins, are components of TATA-binding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265kDa nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other. |
| Immunogen | E1A affinity purified p300 protein. |
| Format | State: Liquid Purification: Protein A affinity purified immunoglobulin fraction BufferSystem: 10 mM PBS pH 7.4 with 0.09% Sodium Azide as preservative and 0.2% BSA as stabilizer. |
| Applications | Immunofluorescence. Immunoprecipitation (Native and denatured): use Protein A and Ab at 2 µg/mg protein lysate. Immunohistology (Formalin/paraffin; 2-4 µg/ml 30 Mins. RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: Ls174T Cells, Tonsil). |
| Specificity | This antibody recognizes p300 as well as CBP. p300 and CBP differ by three noncontiguous residues within the 21 amino acid epitope region, a difference that does not detectably affect the reactivity of NM11 MAb. Epitope is between aa 2071-2091. Cellular Localization: nuclear. Mol. Wt. of Antigen: 300kD Species: Human, Monkey, Mink, Mouse, and Rat. |
| Storage | Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch. |
| References | 1. Dallas PB, et. al. Journal of Virology, 1997, 71(2):1726-31.
2. Dallas PB, et. al. Hybridoma, 1997, 16:273-5. |
| Pictures | Formalin-fixed, paraffin-embedded human tonsil stained with p300 Ab (Cat. AM00262) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00262PU-N p300'.
