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Formalin-fixed, paraffin-embedded human prostate stained with Androgen Receptor Ab (Cat.AM00244PU-N) using peroxidase-conjugate and DAB chromogen. Note nuclear staining of glandular epithelial cells.

 

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AM00244PU-N  Androgen Receptor / AR antibody

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see all 36 Androgen Receptor / AR products
0.1 mg / no delivery possible
please contact the manufacturer

Quick Overview

Mouse anti Androgen Receptor / AR AR441

Synonyms

Dihydrotestosterone Receptor, DHTR, NR3C4, , Nuclear receptor subfamily 3 group C member 4, testicular feminization, spinal and bulbar muscular atrophy, Kennedy disease, AIS, HUMARA, KD, SBMA, SMAX1, TFM

Product review
Please read our product review about Androgen Receptor / AR : Antibodies to Androgen Receptor.

Product Description

Mouse anti Androgen Receptor / AR AR441, Presentation: Purified. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsFrozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityCanine ( Can ), Human ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
CloneAR441
Catalog NumberAM00244PU-N
Swiss Prot. No.P10275
Quantity0.1 mg
Priceno delivery possible
DeliveryNot USA/Canada
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AM00244PU-N.pdf

Datasheet Extract

Concentration0.2 mg/ml
BackgroundThe expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness.
ImmunogenA synthetic peptide from human AR
Epitope: aa 299-315
Swiss Num.: P10275
Format
State: Liquid purified immunoglobulin fraction
Purification: Protein G affinity chromatography
BufferSystem: 10 mM PBS buffer with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer
ApplicationsImmunofluorescence.
Immunoprecipitation (Denatured): use Protein G and antibody at 2 µg/mg protein lysate.
Western Blotting: 1 µg/ml for 2 hrs at RT.
Immunohistochemistry on frozen and paraffin embedded sections: 2-4 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0.
Recommended positive control: LnCap Cells or Prostate Carcinoma.
SpecificityHighly specific for AR.
Cellular Localization: nuclear.
Mol. Wt. of Antigen: 110 kD.
Species: Human and Dog. Does not react with Mouse.
StorageStore at 2-8°C for up to one month or in aliquots at -20°C for longer.
Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch.
References1. Ruizeveld de Winter J A, et al. (1991) J Histochem Cytochem 39: 927-936.
2. Chodak G w, et al. (1992) J Urol 147: 798-803.
PicturesFormalin-fixed, paraffin-embedded human prostate stained with Androgen Receptor Ab (Cat.AM00244PU-N) using peroxidase-conjugate and DAB chromogen. Note nuclear staining of glandular epithelial cells.

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

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