AM00233PU-N DNA PKcs / PRKDC / XRCC7 (N-term) antibody
see related secondary antibodiessee all 37 DNA PKcs / PRKDC / XRCC7 products
0.1 mg / no delivery possible
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Quick Overview
Mouse anti DNA PKcs / PRKDC / XRCC7 18-2
Synonyms
DNA-PKcs, DNA-PK, HYRC, HYRC1, DNPK1, p460, , DNA dependent protein kinase catalytic subunit, DNA PK catalytic subunit antibody, Protein Kinase DNA Activated Catalytic Polypeptide, DNA-PK catalytic subunit
Product review
Please read our product review about DNA PKcs / PRKDC / XRCC7 : Antibodies to XRCC Proteins.
Product Description
Mouse anti DNA PKcs / PRKDC / XRCC7 18-2, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 18-2 |
| Catalog Number | AM00233PU-N |
| Swiss Prot. No. | P78527 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00233PU-N.pdf |
Datasheet Extract
| Monoclonal Antibody to DNA-PKcs | |
| Concentration | 0.2 mg/ml |
| Background | DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks. |
| Immunogen | Human DNA-dependent Protein Kinase purified from HeLa Cells.
Swiss Num.: P78527 |
| Format | State: Liquid purified immunoglobulin fraction Purification: Protein G affinity chromatography BufferSystem: 10 mM PBS with 0.09% Sodium Azide as preservative and 0.2% BSA as stabilizer. |
| Applications | Inhibits (50%) DNA-dependent Phosphorylation.
Immunofluorescence. Immunoprecipitation (Native and denatured): use Protein G and Ab at 2 µg/mg protein lysate. Western Blotting (1-2 µg/ml for 2hrs at RT). Immunohistology (Formalin/paraffin): 2-4 µg/ml for 30 min at RT; REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0. Recommended positive control: LS174T Cells, Tonsil. |
| Specificity | In Western Blotting, this antibody reacts with intact DNA-PKcs as well as its degradation products. It inhibits ~50% of the kinase activity of DNA-PKcs. Preincubation of enzyme with DNA protects DNA-PKcs against inactivation by the antibody.
Cellular Localization: nuclear. Mol. Wt of Antigen: 460 kD. Species: Human and Rat. |
| Storage | Store at 2-8°C for up to one month or in aliquots at -20°C for longer.
Avoid repeated freeze thaw cycles! Shelf life: one year from despatch. |
| References | 1. Carter T; et al. Mol Cell Biol, 1990, 10:6460-71.
2. Han Z; et al. J Biol Chem, 1996 271:25035-40. 3. Kirchgessner CU; et al. Science, 1995, 267(5201):1178-83. 4. Poltoratsky VP; et al. J Immunol, 1995, 155(10):4529-33. 5. Konno-Sato S; et al. Biochem Mol Biol Intl, 1993 31:113-24 |
| Pictures | Formalin-fixed, paraffin-embedded human tonsil stained with DNA-PKcs Ab (Cat. AM00233) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00233PU-N DNA PKcs / PRKDC / XRCC7 '.