AM00223PU-N Ku70 / XRCC6 and Ku80 / XRCC5 antibody
see related secondary antibodiessee all 3 Ku70 / XRCC6 and Ku80 / XRCC5 products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti Ku70 / XRCC6 and Ku80 / XRCC5 162
Synonyms
DNA-repair protein XRCC5, Ku86, TLAA, CTCBF, CTC85, ,
Product review
Please read our product review about Ku70 / XRCC6 and Ku80 / XRCC5: Antibodies to XRCC Proteins.
Product Description
Mouse anti Ku70 / XRCC6 and Ku80 / XRCC5 162, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunofluorescence ( IF ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Immunofluorescence ( IF ), Paraffin Sections ( P ) |
| Reactivity | Human ( Hu ), Monkey ( Mky ), Xenopus ( Xen ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | 162 |
| Catalog Number | AM00223PU-N |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00223PU-N.pdf |
Datasheet Extract
| Monoclonal Antibody to Ku (p70 / p80) | |
| Concentration | 0.2 mg/ml |
| Background | The Ku autoantigen is a heterodimer of 70 kD (p70) and 80 kD (p80) proteins. The p70/p80 dimer is important for function of a 460 kD DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T-antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. |
| Immunogen | Human B-cell nuclei from Plasmacytoid 2p68 Cells. |
| Format | State: Liquid Purification: IgG purified from the ascites fluid by ammonium sulfate precipitation BufferSystem: 10 mM PBS with 0.09 % sodium azide as preservative and 0.2% BSA as stabilizer. |
| Applications | Flow Cytometry. Immunofluorescence. Immunoprecipitation (Native): use Protein A and Ab at 2 µg/mg protein lysate. Not suitable for Western Blotting. Immunohistology (Formalin/paraffin): Ab 1-2µg/ml for 30 min at RT. Recommended positive control: HeLa Cells, Tonsil. |
| Specificity | Reacts with a conformational epitope present on p70/p80 dimer, which is destroyed during Western blotting. Reacts with Mouse and Rat, but verfied in immunoprecipitation only. Cellular Localization: nuclear. Mol. Wt. of Antigen: 70kD and 80kD. Species: Human, Monkey, and Xenopus. Does not react with with Bovine and Rabbit |
| Storage | Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch. |
| References | 1. Wang J, et. al. Molecular Biology Reports,1993, 18(1):15-28.
2. Reeves WH, et. al. Journal of Experimental Medicine 1985 161(1):18-39. |
| Pictures | Formalin-fixed, paraffin-embedded human tonsil stained with Ku Ab (Cat. AM00223) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00223PU-N Ku70 / XRCC6 and Ku80 / XRCC5'.