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Western blot analysis of IκBα in Jurkat cells treated with (right lane) or without (left lane) TNF-α (1 nM).

 

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AM00179PU-N  I Kappa B alpha (IKBa) antibody

see related secondary antibodies
see all 19 I Kappa B alpha products
0.1 mg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti I Kappa B alpha (IKBa) 6A055

Product Description

Mouse anti I Kappa B alpha (IKBa) 6A055, Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )

Properties

ApplicationsWestern blot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
Clone6A055
Catalog NumberAM00179PU-N
Quantity0.1 mg
PriceUS$ 425
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
AM00179PU-N.pdf

Datasheet Extract

Monoclonal Antibody to I kappa B alpha
Concentration0.25 mg/ml
BackgroundNF-κB is silenced in the cytoplasm by an inhibitory protein, IκB. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/γ. External stimuli such TNF-α or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Six members of IκB family members have been identified. One of the first gene induced following NF-κB activation is IκBα.
ImmunogenRecombinant human IκBα
Format
State: Liquid purified IgG
Purification: Immunoaffinity
BufferSystem: PBS, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal
ApplicationsWestern Blot: 0.5-4 μg/ml.
Immunoprecipitation: 1-4 μg/ml.
SpecificityThe antibody detects both phosphorylated and non-phosphorylated forms of IκBα.
Species: Human, Mouse.
StorageStore the antibody undiluted at -20°C or for long term storage (in aliquots) at -70°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
PicturesWestern blot analysis of IκBα in Jurkat cells treated with (right lane) or without (left lane) TNF-α (1 nM).

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'AM00179PU-N I Kappa B alpha (IKBa)'.