AM00078PU-N CD220 / INSR / Insulin Receptor beta (C-term) (incl. pos. control) antibody
see related secondary antibodiessee all 8 CD220 / INSR / Insulin Receptor beta products
0.1 mg / no delivery possible
please contact the manufacturer
Quick Overview
Mouse anti CD220 / INSR / Insulin Receptor beta 11B6
Synonyms
INSR, CD220, IR, EC 2.7.10.1, Insulin receptor, , IGFR1, Insulin growth factor 1 receptor, CD221
Product review
Please read our product review about CD220 / INSR / Insulin Receptor beta: Antibody Panel to Insulin Receptor (CD220).
Product Description
Mouse anti CD220 / INSR / Insulin Receptor beta 11B6, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Reactivity | Canine ( Can ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 11B6 |
| Catalog Number | AM00078PU-N |
| Swiss Prot. No. | P06213 |
| Quantity | 0.1 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  AM00078PU-N.pdf |
Datasheet Extract
| Background | The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes. |
| Immunogen | Synthetic peptide conjugated to KLH. Swiss Num.: P06213 |
| Format | State: Lyophilized purified IgG Purification: Subsequent thiophilic adsorption and size exclusion chromatography BufferSystem: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose Reconstitution: Restore with 1 ml H2O (15 min, RT). |
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 µg/ml. |
| Specificity | This antibody specifically recognizes the C-terminus of Insulin receptor (phosphorylation-independent). Species: Human, Mouse, Rat, Dog. |
| Add. Information | Mol. Weight: 97 kDa. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20 to -80°C.
Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months. Shelf life: one year from despatch. |
| Protocols | Positive Control Cell Lysate
Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Recostitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freezing and thawing. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. |
| Pictures | Detection of endogenous InsR Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-11B6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'AM00078PU-N CD220 / INSR / Insulin Receptor beta'.
